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anti mouse eomesodermin  (Miltenyi Biotec)
90
Miltenyi Biotec anti mouse eomesodermin
Expression of transcription factors in tumor antigen‐specific CD8 + T cells in the immune control effector phase. B16OVA‐Luc2 cells (10 5 cells/mouse) were used to sc inoculate vaccinated C57BL/6 mice. B16OVA‐Luc2 cells (10 5 cells/mouse) were used to sc inoculate vaccinated C57BL/6 mice. At the indicated time‐points, TIL were collected and subjected to flow cytometry (Day 0 = tumor inoculation). Representative histograms (left) and summaries (right) of <t>Eomes</t> (A) and T‐bet (B) expression in OVATet + T cells are shown. C, Representative contour plots (left) and summaries (middle) of TOX expression (TOX hi ) on OVATet + T cells in TIL are shown. The percentage of TOX hi OVATet + T cells isolated from the indicated sources is shown (right). Data are presented as the mean ± SEM
Anti Mouse Eomesodermin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression of transcription factors in tumor antigen‐specific CD8 + T cells in the immune control effector phase. B16OVA‐Luc2 cells (10 5 cells/mouse) were used to sc inoculate vaccinated C57BL/6 mice. B16OVA‐Luc2 cells (10 5 cells/mouse) were used to sc inoculate vaccinated C57BL/6 mice. At the indicated time‐points, TIL were collected and subjected to flow cytometry (Day 0 = tumor inoculation). Representative histograms (left) and summaries (right) of Eomes (A) and T‐bet (B) expression in OVATet + T cells are shown. C, Representative contour plots (left) and summaries (middle) of TOX expression (TOX hi ) on OVATet + T cells in TIL are shown. The percentage of TOX hi OVATet + T cells isolated from the indicated sources is shown (right). Data are presented as the mean ± SEM

Journal: Cancer Science

Article Title: NKG2D defines tumor‐reacting effector CD8 + T cells within tumor microenvironment

doi: 10.1111/cas.15050

Figure Lengend Snippet: Expression of transcription factors in tumor antigen‐specific CD8 + T cells in the immune control effector phase. B16OVA‐Luc2 cells (10 5 cells/mouse) were used to sc inoculate vaccinated C57BL/6 mice. B16OVA‐Luc2 cells (10 5 cells/mouse) were used to sc inoculate vaccinated C57BL/6 mice. At the indicated time‐points, TIL were collected and subjected to flow cytometry (Day 0 = tumor inoculation). Representative histograms (left) and summaries (right) of Eomes (A) and T‐bet (B) expression in OVATet + T cells are shown. C, Representative contour plots (left) and summaries (middle) of TOX expression (TOX hi ) on OVATet + T cells in TIL are shown. The percentage of TOX hi OVATet + T cells isolated from the indicated sources is shown (right). Data are presented as the mean ± SEM

Article Snippet: For intracellular staining, cells were fixed and permeabilized in accordance with the manufacturer's instructions (FoxP3/Transcription factor staining buffer set, eBioscence) before being stained with fluorophore‐labeled antibodies: anti‐mouse eomesodermin (Eomes, clone REA116, MACS Miltenyi Biotec), T‐bet (4B10, BioLegend), and TOX (REA473, Miltenyi Biotec).

Techniques: Expressing, Control, Flow Cytometry, Isolation